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recombinant human p selectin cd62p fc chimera  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human p selectin cd62p fc chimera
    Recombinant Human P Selectin Cd62p Fc Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+selectin+fc+chimera/Recombinant+Human+P-Selectin%2FCD62P+Fc+Chimera+Protein%2C+CF/pm41168811-72-0-5
    Average 93 stars, based on 40 article reviews
    recombinant human p selectin cd62p fc chimera - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome.
    Article Snippet: HUVECs were pelleted by centrifugation, washed twice with BD Perm/Wash buffer (BD Pharmingen), stained with PE-conjugated rabbit anti-activated caspase-3 antibody (BD Pharmingen) in BD Perm/Wash buffer for 30 min at room temperature, and analyzed by flow cytometry. .. To examine selectin binding, PBMCs were stimulated with and without the anti-human CD3 monoclonal antibody Orthoclone OKT3 (Janssen Pharmaceutical, Tokyo, Japan) for 48 h, then incubated with 50 ng/μL recombinant human E- or P-selectin/Fc chimera (R&D Systems, Minneapolis, MN, USA). .. Cells were then labeled with IgG F1-FITC (Sigma-Aldrich, St. Louis, MO, USA), CD4-PE-Cy5, CD69-FITC, or CD162-PE monoclonal antibodies followed by flow cytometric analysis.

    Incubation:

    Article Title: P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome.
    Article Snippet: HUVECs were pelleted by centrifugation, washed twice with BD Perm/Wash buffer (BD Pharmingen), stained with PE-conjugated rabbit anti-activated caspase-3 antibody (BD Pharmingen) in BD Perm/Wash buffer for 30 min at room temperature, and analyzed by flow cytometry. .. To examine selectin binding, PBMCs were stimulated with and without the anti-human CD3 monoclonal antibody Orthoclone OKT3 (Janssen Pharmaceutical, Tokyo, Japan) for 48 h, then incubated with 50 ng/μL recombinant human E- or P-selectin/Fc chimera (R&D Systems, Minneapolis, MN, USA). .. Cells were then labeled with IgG F1-FITC (Sigma-Aldrich, St. Louis, MO, USA), CD4-PE-Cy5, CD69-FITC, or CD162-PE monoclonal antibodies followed by flow cytometric analysis.

    Article Title: T lymphocytes migrate upstream after completing the leukocyte adhesion cascade
    Article Snippet: .. Next, a solution of ICAM-1/Fc and/or VCAM-1/Fc and P-selectin/Fc chimera (R&D Systems, Cat. 720-IC, 862-VC, 137-PS, respectively) was applied to the surface and incubated for 3 h at room temperature. ..

    Recombinant:

    Article Title: P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome.
    Article Snippet: HUVECs were pelleted by centrifugation, washed twice with BD Perm/Wash buffer (BD Pharmingen), stained with PE-conjugated rabbit anti-activated caspase-3 antibody (BD Pharmingen) in BD Perm/Wash buffer for 30 min at room temperature, and analyzed by flow cytometry. .. To examine selectin binding, PBMCs were stimulated with and without the anti-human CD3 monoclonal antibody Orthoclone OKT3 (Janssen Pharmaceutical, Tokyo, Japan) for 48 h, then incubated with 50 ng/μL recombinant human E- or P-selectin/Fc chimera (R&D Systems, Minneapolis, MN, USA). .. Cells were then labeled with IgG F1-FITC (Sigma-Aldrich, St. Louis, MO, USA), CD4-PE-Cy5, CD69-FITC, or CD162-PE monoclonal antibodies followed by flow cytometric analysis.

    Fluorescence:

    Article Title: Cell adhesion molecules in metastatic neuroblastoma models.
    Article Snippet: Several cell adhesion molecules (CAMs) including selectins, integrins, cadherins and immunoglobulin-like CAMs are involved in leukocyte adhesion especially at sites of inflammation.. In cancer cells, these CAMs have been associated with the growth and metastatic behavior in several malignant entities.. In this study adhesion of LAN 1 and SK-N-SH neuroblastoma cells to selectins, hyaluronan and endothelial cells were determined under flow conditions.

    FACS:

    Article Title: Cell adhesion molecules in metastatic neuroblastoma models.
    Article Snippet: Several cell adhesion molecules (CAMs) including selectins, integrins, cadherins and immunoglobulin-like CAMs are involved in leukocyte adhesion especially at sites of inflammation.. In cancer cells, these CAMs have been associated with the growth and metastatic behavior in several malignant entities.. In this study adhesion of LAN 1 and SK-N-SH neuroblastoma cells to selectins, hyaluronan and endothelial cells were determined under flow conditions.

    Binding Inhibition:

    Article Title: E-Selectin Inhibition Mitigates Splenic HSC Activation and Myelopoiesis in Hypercholesterolemic Mice With Myocardial Infarction
    Article Snippet: The absorbance at 450 nm was measured using a FlexSation 3 (Molecular Devices). .. The P-selectin binding-inhibition assay was similar to the Eselectin assay, except the P-selectin-Fc chimera (R&D Systems 137-PS) was captured with anti-human IgG antibody. ..



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